DETECTION OF NITROGENASE PRODUCING BACTERIA FROM THE SOIL OF LIWA BOTANICAL GARDEN

Authors

  • Cristina Nugroho Ekowati
  • Mica Mirani Jurusan Biologi FMIPA Universitas Lampung
  • Kusuma Handayani Jurusan Biologi FMIPA Universitas Lampung
  • Rochmah Agustrina

DOI:

https://doi.org/10.23960/jbekh.v8i2.204

Keywords:

KRL, NFB, Nitrogen, Nitrogenase

Abstract

Liwa Botanical Gardens is an ex-situ conservation area for various types of plants. Each plant produces organic matter that will provide nutrients for the growth of nitrogen-fixing bacteria. This indicates the existence of an environment that supports the growth of nitrogen-fixing bacteria. Nitrogen is one of the nutrients needed by plants for their growth. However, the abundance of nitrogen in the atmosphere cannot be utilized directly by plants but needs to transform into ammonium and nitrate first. This transformation can be done by nitrogen-fixing bacteria through an enzymatic process. This research aims to obtain bacterial isolates that can fix nitrogen. Nitrogen-fixing bacteria were isolated using Nutrient Agar (NA) medium and furthered by nitrogenase activity detection test with semi-solid Nitrogen Free Bromothymol Blue (NFB). Nitrogen-fixing bacteria are characterized by color changes in the medium. The results obtained 22 isolates with 3 isolates detected capable of producing nitrogenase enzymes, namely TBP B3, TB1 B2, and TMA2 B2.

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Published

2021-12-30

How to Cite

Ekowati, C. N., Mirani, M., Handayani, K., & Agustrina, R. (2021). DETECTION OF NITROGENASE PRODUCING BACTERIA FROM THE SOIL OF LIWA BOTANICAL GARDEN. Jurnal Ilmiah Biologi Eksperimen Dan Keanekaragaman Hayati (J-BEKH), 8(2), 53–58. https://doi.org/10.23960/jbekh.v8i2.204

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