Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh <h2><a style="background-color: #ffffff; font-size: 14px;" href="http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/management/settings/jurnalbiologi.fmipa.unila.ac.id/index.php/solma/announcement/view/9">INFORMATION</a></h2> <article class="obj_announcement_summary"> <div class="summary"> <ol> <li class="show"><strong>Journal Name: Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) </strong></li> <li class="show"><strong>Print ISSN</strong><strong>:</strong> <strong><a href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&amp;1370585899&amp;1&amp;&amp;">2338-4344</a></strong></li> <li class="show"><strong>Online ISSN</strong><strong>:</strong> <strong><a href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&amp;1568186656&amp;1&amp;&amp;">2686-200X</a></strong></li> <li class="show"><strong>Frequency</strong><strong>: </strong>2 issue per tahun (<strong>Juni</strong> dan <strong>Nopember</strong>)</li> <li class="show"><strong>Total article:</strong> 10-12 artikel per issue</li> <li class="show"><strong>DO</strong><strong>I:</strong> <strong><a href="https://search.crossref.org/?q=2614-1531">10.23960</a></strong></li> <li class="show"><strong>Editor in Chief:</strong> <strong><a href="https://scholar.google.co.id/citations?user=BOZ3GE8AAAAJ&amp;hl=en">Dr. Bambang Irawan, M.Sc.</a></strong></li> <li class="show"><strong>Email Journal: </strong>achmad.arifiyanto@fmipa.unila.ac.id</li> <li class="show"><strong>Website:</strong><a title="Halaman website" href="https://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh"> http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh</a></li> <li class="show"><strong>Publisher</strong><strong>:</strong> Jurusan Biologi Fakultas MIPA Universitas Lampung bekerjasama dengan Perhimpunan Biologi Indonesia</li> <li class="show"><strong>Country:</strong> Indonesia</li> <li class="show"><strong>Language:</strong> Indonesian and English.</li> <li class="show"><strong>Open Access:</strong> Ya</li> <li class="show"><strong>Article Publication Charge (APC) :</strong> Ya</li> <li class="show"><strong>Review:</strong> Double Blind Peer Review</li> <li class="show"><strong>Recommended Tools:</strong> Ya (<strong>Crossref Similarity Check (iThenticate)</strong><strong> dan</strong> <strong>Grammarly)</strong></li> <li class="show"><strong>Full-text Archives:</strong> <a href="http://www.lockss.org/">LOCKSS</a> and <a href="http://clockss.org/">CLOCKSS</a></li> <li><strong>Science and Technology <em>Index</em> (<em>SINTA</em>):</strong> Terindeks <a href="https://sinta.kemdikbud.go.id/journals/detail?id=8468">S-4 </a>berdasarkan Hasil Akreditasi Jurnal Ilmiah Periode II Tahun 2021 dan telah diterbitkannya Surat Keputusan Direktur Jenderal Pendidikan Tinggi, Riset, dan Teknologi Nomor 164/E/KPT/2021, tanggal 27 Desember 2021 tentang Peringkat Akreditasi Jurnal Ilmiah periode II Tahun 2021.</li> </ol> </div> </article> Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch. en-US Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) 2338-4344 The Diversity of Singing Birds at the Way Canguk Research Station, Bukit Barisan Selatan National Park http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/article/view/206 <p>Birds have an important role in controlling natural ecosystems. The presence of hunting traces every year indicates hunting at the Way Canguk Research Station. If this happens continuously, it can cause a decrease in the diversity and population of chirping birds. Therefore, this research needs to be carried out to determine the diversity, evenness, richness, relative density, as well as environmental factors that affect the chirping bird community, and the intensity of bird hunting in SPWC. The research was conducted at the Way Canguk Research Station, Bukit Barisan Selatan National Park, using the point count method.</p> Agista Andriyani Nuning Nurcahyani Gregorius Nugroho Susanto Marsya Christyanti Sibarani Laji Utoyo Copyright (c) 2022 Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) https://creativecommons.org/licenses/by-sa/4.0 2022-01-06 2022-01-06 9 1 1 11 10.23960/jbekh.v9i1.206 Growth of Hornbill Feed Seeds at Way Canguk Research Station, Bukit Barisan Selatan National Park http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/article/view/207 <table> <tbody> <tr> <td width="300"> <p>Rangkong merupakan jenis burung pemakan buah (<em>frugivory</em>). Buah yang dikonsumsi rangkong dikategorikandalam buah yang kecil dengan jumlah yang banyakdan jenis buah yang memiliki batu (<em>stone seeds</em>), yaitu jenis fikus dan non fikus. Peran rangkong di hutan sangatlah penting yaitu sebagai penebar bijimelalui sisa makanan dan kotoran rangkong karena sistem pencernaannya yang tidak merusak biji sehingga mencerminkan hutan yang sehat dan menandakan masih adanya pohon-pohon besar di wilayah tersebut. Penelitian ini dilakukan di Stasiun Penelitian Way Canguk (SPWC) TNBBS Lampung dengan metode observasi langsung melalui pengamatan laju pertumbuhan biji pakan rangkong secara generatif (semai) pada lokasi yang berbeda. Data disajikandalam bentuk tabel dan diagram kemudian dianalisis secara deskriptif. Hasil penelitian menunjukan diperolehnya biji sebanyak 581 buah dari 12 famili dan 26 spesies serta 7 jenis yang belum teridentifikasi. Nasib biji setelah dimuntahkan tumbuh sebanyak 39% dari keseluruhan biji yang diperoleh dan 61% biji tidak mengalami pertumbuhan diduga karena adanya predator biji dan rusaknya biji serta faktor naungan yang dapat menghambat pertumbuhan biji. Biji dari buah fikus yang berasal dari defekasi tidak mengalami pertumbuhan. Nilai laju pertumbuhan semai di alam lebih cepat dibandingkan dengan semai yang berada di media terkontrol atau disekitar kamp SPWC.</p> <p> </p> <p> </p> </td> <td width="300"> <p><em>The hornbill is a type of frugivory bird. The fruit consumed by the hornbill is categorized into small fruit with a large number and the type of fruit that has stone seeds, namely ficus and non-ficus types. The role of hornbill in the forest is very important, namely as seed dispersal through food waste and hornbill droppings because their digestive system does not damage the seeds so that it reflects a healthy forest and indicates the presence of large trees in the area. This research was conducted at the Way Canguk Research Station (SPWC) TNBBS Lampung with direct observation by observing the growth rate of generative hornbill feed seeds (seedlings) at different locations. The data is presented in the form of tables and diagrams and then analyzed descriptively. The results showed that 581 seeds were obtained from 12 families and 26 species and 7 species that had not been identified. The fate of seeds after vomiting grew as much as 39% of the total seeds obtained and 61% of seeds did not experience growth, presumably due to seed predators and seed damage and shading factors that could inhibit seed growth. Seeds of ficus fruit from defecation did not grow. The value of the growth rate of seedlings in nature was faster than those in controlled media or around the SPWC camp.</em></p> <p><em> </em></p> <p> </p> <p><em> </em></p> </td> </tr> </tbody> </table> Dimaz Irawan Nuning Nurcahyani Priyambodo . M Kanedi Laji Utoyo Copyright (c) 2022 Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) https://creativecommons.org/licenses/by-sa/4.0 2022-01-06 2022-01-06 9 1 12 23 10.23960/jbekh.v9i1.207 Potentially Invasive Plant Types in Way Kambas National Park http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/article/view/208 <table width="601"> <tbody> <tr> <td width="300"> <p>Tumbuhan asing invasif merupakan salah satu ancaman bagi keanekaragaman hayati Indonesia. Tumbuhan ini dapat mengubah ekosistem dengan mendominasi lanskap. Mengingat besarnya dampak yang dapat ditimbulkan oleh invasi tumbuhan di kawasan konservasi, maka perlu dilakukan inventarisasi jenis-jenis tumbuhan yang berpotensi invasif di Taman Nasional Way Kambas (TNWK), sehingga dampak negatif dari invasi akan mudah di cegah. Inventarisasi dilakukan dengan membuat plot analisis vegetasi pada empat tipe vegetasi di TNWK, yaitu vegetasi hutan, vegetasi hutan campuran, vegetasi rawa dan vegetasi alang-alang. Berdasarkan hasil analisis vegetasi, diperoleh 11 jenis tumbuhan berpotensi invasif di TNWK, yaitu <em>Actinoscirpus grossus</em>, <em>Clidemia hirta</em>, <em>Chromolaena odorata</em>, <em>Fimbristylis ovata</em>, <em>Hyptis capitata</em>, <em>Imperata cylindrica</em>, <em>Isachne globosa</em>, <em>Lantana camara</em>, <em>Melaleuca leucadendron</em>, <em>Melastoma malabathricum</em>, dan <em>Mimosa pigra</em>.</p> <p> </p> <p> </p> </td> <td width="300"> <p><em>Invasive alien plant species are a threat to Indonesia's biodiversity. These plants can change ecosystems by dominating landscapes. Given the magnitude of the impact that can be caused by plant invasions in conservation areas, it is necessary to carry out an inventory of potentially invasive plant species in Way Kambas National Park (TNWK), so that the negative impacts of these invasions can be easily prevented. . The inventory was carried out by plotting vegetation analysis on four types of vegetation in TNWK, namely forest vegetation, mixed forest vegetation, swamp vegetation, and alang-alang vegetation. Based on the results of the vegetation analysis, 11 species of plants that have the potential to be invasive in TNWK were found, namely </em>Actinoscirpus grossus, Clidemia hirta, Chromolaena odorata, Fimbristylis ovata, Hyptis capitata, Imperata cylindrica, Isachne globosa, Lantana camara, Melaleuca leucadendron, Melastoma malabathricum, dan Mimosa pigra<em>.</em></p> <p><em> </em></p> <p> </p> <p> </p> </td> </tr> </tbody> </table> Jani Master Sumianto Santoso Ahmad Fanani Nur Alim Ichan Prastika Muhammad Yunus Copyright (c) 2022 Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) https://creativecommons.org/licenses/by-sa/4.0 2022-01-06 2022-01-06 9 1 24 33 10.23960/jbekh.v9i1.208 Eating Behavior of the Klihingan Hornbill (Anorrhinus Galeritus) When Nesting at the Way Canguk Research Station, Bukit Barisan Selatan National Park (TNBBS) http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/article/view/217 <table> <tbody> <tr> <td width="300"> <p>Enggang klihingan merupakan salah satu jenis rangkong (Bucerotidae) yang hidup berkelompok, berperan penting secara ekologis sebagai penyebar biji. Perilaku bersarangnya unik, selama mengerami induk betina mengurung diri di lubang pohon yang ditutup lumpur dan disisakan lubang kecil untuk enggang lainnya melewatkan pakan dari luar sarang. Penelitian ini bertujuan untuk mengetahui perilaku makan enggang pada saat bersarang dan mengumpulkan data jenis buah pakannya. Penelitian dilakukan menggunakan metode <em>focal animal sampling</em>, pada bulan Februari – April 2021 di Stasiun Penelitian Way Canguk (SPWC), Taman Nasional Bukit Barisan Selatan (TNBBS). Perilaku enggang yang teramati dominan berupa mengeluarkan satu per satu pakan dari dalam temboloknya, kemudian memosisikan pakan ke ujung paruh untuk dimuntahkan ke sarang. Komposisi buah yang dijadikan pakan enggang meliputi 18 jenis buah, yaitu <em>Ficus altissima, Ficus sundaica, Ficus crassiramea, Ficus stupenda var.minor, Polyalthia lateriflora, Oncosperma horridum, Canarium megalanthum, Canthiumera glabra, Zuccarinia macrophylla, Antiaris toxicaria, Myristica sp., Horsfieldia sp., Magnolia champaca, Dysoxylumsp., Fibraurea tinctoria, Endocomia macrocoma, Actinodhapne borneensis</em>, dan <em>Alseodaphne falcata</em>. Waktu yang paling sering digunakan untuk memberi makan adalah sore hari pukul 16.00 – 17.00 WIB. Rerata enggang mengunjungi sarang 15 kali/hari dan membawa paling banyak 16 buah dalam sekali kunjungan. Enggang mampu memberi makan dengan lama durasi 3 – 5 buah/menit.</p> <p> </p> <p> </p> </td> <td width="300"> <p><em>Bushy-crested hornbill is a type of hornbill (Bucerotidae) that lives in groups, playing an important ecological role. Their nesting behavior is unique, during incubation, the female locks herself in a mud-covered tree hole and leaves a small hole for other hornbills to spread food from outside the nest. This study aimed to find out the eating behavior of hornbills during nesting and collect data on the types of fruit they eat. This study was conducted using a focused animal sampling method, from February to April 2021 at Way Canguk Research Station (SPWC), Bukit Barisan Selatan National Park (TNBBS). The dominant behavior of hornbills was observed in the form of removing the bait one by one from the cache, then placing the bait to the tip of the beak to vomit into the nest. The fruit composition used as hornbill food includes 18 types of fruit, namely </em>Ficus altissima, Ficus sundaica, Ficus crassiramea, Ficus stupenda <em>var</em>. minor, Polyalthia lateriflora, Oncosperma horridum, Canarium megalanthum, Canthiumera glabra, Zuccarinia macrophylla, Antiaris toxicaria, Myristica <em>sp</em>., Horsfieldia <em>sp</em>., Magnolia champaca, Dysoxylum <em>sp</em>., Fibraurea tinctoria, Endocomia macrocoma, <em>and</em> Alseodaphne falcata.<em> The most common time used for feeding is from 16:00 - 17:00 WIB. The average hornbill visits the nest 15 times/day and brings a maximum of 16 fruits in one visit. Hornbills can feed with a fairly low duration (3-5 pieces/minute). </em></p> <p><em> </em></p> <p> </p> </td> </tr> </tbody> </table> Messy Apriliasari Nuning Nurcahyani Elly Lestari Rustiati Priyambodo Priyambodo Laji Utoyo Copyright (c) 2022 Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) https://creativecommons.org/licenses/by-sa/4.0 2022-01-06 2022-01-06 9 1 34 41 10.23960/jbekh.v9i1.217 Larvicide Effects of Serratia marcescens strain MBC1 Extract on Instar III Larvae of Aedes aegyti http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/article/view/219 <table> <tbody> <tr> <td width="300"> <p>Bakteri merupakan kandidat yang berpotensi sebagai larvasida <em>Aedes aegypti</em>. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak <em>Serratia marcecsens</em> strain MBC1 terhadap kematian larva instar III <em>Aedes aegypti</em>. Penelitian ini merupakan penelitian eksperimental dengan dua macam bentuk pengujian, yaitu uji larvasida untuk menentukan mortalitas dan uji lanjut untuk melihat perkembangan larva setelah perlakuan larvasida. Larva instar III diberi perlakuan dengan konsentrasi 125, 250, 500, dan 1.000 ppm. Uji larvasida diamati selama 24-72 jam. Uji lanjut larva instar III diberi perlakuan dengan konsentrasi 500 dan 1.000 ppm serta diamati sampai semua larva mati. Kontrol positif menggunakan Abate® 1% dan kontrol negatif menggunakan air sumur. Hasil analisis probit didapatkan bahwa nilai LC50 dan LC90 ekstrak <em>S</em>. <em>marcescens</em> adalah 66.426,02 dan 749.001,41ppm. Larva instar III mati pada 18 hari setelah perlakuan ekstrak <em>S</em>. <em>marcescens</em> strain MBC1. Hasil dari penelitian ini menunjukkan bahwa larvasida ekstrak <em>S</em>. <em>marcescens</em> mempengaruhi lamanya siklus hidup dan mati sebelum mencapai stadium dewasa.</p> <p> </p> <p> </p> </td> <td width="300"> <p><em>Bacteria have potencial as larvicide of </em>Aedes aegypti.<em> The purpose of this study is to determine the effect of </em>Serratia marcescens<em> strain MBC1 against larval third instars of Aedes aegypti. This research was an experimental study with two kinds of testing. The larvicide test to determine the mortality of the larval and the continuous test to observe the development of the larval after treatment with the larvacide. The third instars of mosquito larval were given bacterial extract in four concentrations (125, 250, 500, and 1000 ppm), and observed mortality during 72 hours. The continuous larvicide test was observed at two concentrations (500 and 1,000 ppm). The observation is completed when all of the larvae died. The positive control used Abate® 1% and the negative control used natural water. The treatment was repeated 4 times. The results show that LC50 and LC90 of </em>S. marcescens<em> extract are 66,426.02 dan 749,001.41 ppm. All of the larvae in the continuous test die after 18 days have been givenan extract of S. marcescens strain MBC1. The experiment shows that the larvicide of extract of </em>S. marcescens<em> influences the length of the life cycle and larvae of </em>Ae. aegypti <em>die before reaching the adult.</em></p> <p><em> </em></p> <p> </p> </td> </tr> </tbody> </table> Mutia Dinda Lestari Nismah Nukmal Endah Setyaningrum Salman Farisi Achmad Arifiyanto Copyright (c) 2022 Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) https://creativecommons.org/licenses/by-sa/4.0 2022-01-06 2022-01-06 9 1 42 48 10.23960/jbekh.v9i1.219 Terathogenic Testing of Black Cumin (Nigella sativa L.) Extract on The Number of Fetuses, Length of Front and Back Extremities, and The Number of Malformation Fetus in Mice (Mus musculus L.) http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/article/view/218 <table> <tbody> <tr> <td width="300"> <p>Pemakaian obat tradisional telah berkembang dengan baik dan saat ini sudah banyak digunakan sebagai obat alternatif untuk mengatasi masalah kesehatan. Obat tradisional akan memberikan khasiat yang baik apabila dikonsumsi dengan cara dan dosis yang tepat untuk tujuan pengobatan. Berdasarkan penelitian sebelumnya pemberian jintan hitam (<em>Nigella sativa </em>L) terhadap mencit betina menyebabkan terjadinya aktivitas antifertilisasi, <em>antinociceptif</em>, kontrasepsi <em>postcoitus</em>, dan antioksitosin yang diduga dapat melancarkan menstruasi. Tingkat keamanan konsumsi jintan hitam pada masa kehamilan terhadap perkembangan fetus, serta sistem reproduksi mencit betina yang menimbulkan malfomasi <em>congenital </em>pada anaknya belum banyak diteliti. Tujuan dari penelitian ini untuk mengetahui pengaruh ekstrak jintan hitam (<em>Nigella sativa </em>L) terhadap jumlah fetus mencit,ukuran panjang ekstremitas depan dan belakang pada fetus mencit, dan jumlah fetus malformasi pada mencit. Penelitian ini menggunakan 20 ekor mencit betina yang sudah bunting dan dibagi menjadi 4 kelompok yaitu kelompok K (kontrol) diberi 0,3 mL aquabides, kelompok perlakuan P<sub>1 </sub>dengan dosis 2,1 mg/g BB dalam 0,3 mL aquabides, kelompok perlakuan P<sub>2 </sub>dengan dosis 8,4 mg/g BB dalam 0,3 mL aquabides, dan kelompok perlakuan P<sub>3 </sub>dengan dosis 33,6 mg/g BB dalam 0,3 mL aquabides. Perlakuan diberikan pada hari ke 6 sampai ke 17 kebuntingan. Hasil menunjukkan bahwa pemberian ekstrak jintan hitam tidak memberikan pengaruh terhadap jumlah fetus yang dikandung dan tidak menurunkan pertumbuhan ekstrimitas depan dan belakang fetus mencit karena tidak menunjukkan hasil yang signifikan antara kelompok kontrol (K) dan kelompok perlakuan (P1, P2, dan P3). Pemberian ekstrak jintan hitam (<em>Nigella sativa L</em>.) tidak meningkatkan jumlah fetus malformasi akan tetapi menyebabkan adanya fetus yang mati pada saat kelahiran dan mengalami malformasi pada beberapa fetus lainnya.</p> <p> </p> <p> </p> </td> <td width="300"> <p><em>The use of traditional medicine has developed well and is now widely used as an alternative medicine to treat health problems. Traditional medicine will provide good efficacy if consumed in the right way and dosed for medicinal purposes. Based on previous research, giving black cumin </em>(Nigella sativa<em> L) to female mice caused infertility, antinociceptive, postcoitus contraception, and anti-oxytocin activities which were thought to be able to launch menstruation. The safety level of consumption of black cumin during pregnancy on fetal development, as well as the reproductive system of female mice that cause congenital malformations in their offspring, has not been widely studied. The purpose of this study was to determine the effect of black cumin extract (</em>Nigella sativa<em> L) on the number of mice fetuses, the length of the front and back extremities in mice fetuses, and the number of malformed fetuses in mice. This study used 20 pregnant female mice and was divided into 4 groups, namely group K (control) was given 0.3 mL aquabidest, treatment group P1 with a dose of 2.1 mg/g BW in 0.3 mL aquabides</em><em>t</em><em>, treatment group P2 with a dose of 8.4 mg/g BW in 0.3 mL aquabidest, and the P3 treatment group with a dose of 33.6 mg/g BW in 0.3 mL aquabidest. The treatment was given on the 6th to 17th day of pregnancy. The results showed that the administration of black cumin extract had no effect on the number of fetuses conceived and did not reduce the growth of the front and rear extremities of the mice fetuses because it did not show significant results between the control group (K) and the treatment group (P1, P2, and P3). The administration of black cumin extract (</em>Nigella sativa<em> L.) did not increase the number of malformed fetuses but caused fetal death at birth and malformations in several other fetuses.</em></p> <p> </p> <p> </p> <p><em> </em></p> </td> </tr> </tbody> </table> Zikra Fardhira Nuning Nurcahyani Rochmah Agustrina Tugiyono Tugiyono Copyright (c) 2022 Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) https://creativecommons.org/licenses/by-sa/4.0 2022-01-06 2022-01-06 9 1 49 56 10.23960/jbekh.v9i1.218 Effectiveness of Ovitrap Against Aedes aegypti Mosquito In Kemiling Raya Sub-District Bandar Lampung City And The Vulnerability Of Its Larvals To Temephos http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/article/view/205 <table> <tbody> <tr> <td width="300"> <p>Demam Berdarah Dengue (DBD) adalah salah satu masalah kesehatan masyarakat, yang disebabkan oleh <em>Virus Dengue</em>. Di Indonesia kasus DBD masih tinggi, khususnya di Lampung, salah satu Kota dengan angka kasus DBD yang masih tinggi adalah Bandar Lampung. Dari berbagai hasil penelitian diketahui ovitrap memiliki fungsi monitoring serta pengendalian <em>Aedes</em> sp. Upaya pengendalian vektor nyamuk <em>Ae.</em> <em>aegypti</em> banyak dilakukan baik secara alami ataupun dengan cara kimia, cara kimia yang paling umum digunakan adalah penggunaan temephos. Penggunaan insektisida kimia seperti temephos secara terus menerus dapat menyebabkan resistensi. Tujuan penelitian untuk mengetahui efektivitas ovitrap di dalam dan luar rumah, mengetahui ovitrap index, serta status kerentanan larva nyamuk <em>Ae.</em> <em>aegypti</em> terhadap temephos di Kelurahan Kemiling Raya Kota Bandar Lampung. Penelitian dilakukan bulan Februari-April 2021. Ovitrap diletakkan di 142 rumah di dalam dan luar rumah, hasil telur dihitung, dipelihara dan diuji kerentananya. Bahan yang digunakan larva instar III hasil pemeliharaan hingga F1. Penelitian dilakukan dengan 4 kali ulangan, menggunakan 25 larva pada tiap gelas dengan larutan temephos 0,02 mg/L dalam 250 mL air dibiarkan kontak uji selama 1 jam dan masa pemulihan selama 24 jam. Hasil yang di dapat 83,10% rumah terdapat telur pada ovitrap dan 16,90% tidak ada telur, jumlah telur pada ovitrap di luar rumah (57%) lebih banyak dari dalam rumah (43%) serta ovitrap index yang didapatkan adalah 72,5%, persentase kematian 99% dan dikategorikan rentan.</p> <p> </p> <p> </p> </td> <td width="300"> <p><em>Dengue Hemorrhagic Fever is one of the public health problems, which is caused by the Dengue Virus. In Indonesia, dengue cases are still high, especially in Lampung, one of the cities with a high number of dengue cases is Bandar Lampung. From various research results, it is known thatovitrap has the function of monitoring and controlling </em>Aedes<em> sp. Efforts to control the mosquito vector of </em>Ae. aegypti<em> is mostly done either naturally or chemically, the most commonly used chemical method is the use of temephos. The continuous use of chemical insecticides such as temephos can cause resistance. The purpose of the study was to determine the effectiveness of ovitrapinside and outside the home, to determine the ovitrap index, and the susceptibility status of </em>Ae. aegypti<em> against temephos in Kemiling Raya Village, Bandar Lampung City. The study was conducted in February-April 2021. Ovitrapswere placed in 142 houses inside and outside the house, egg yields were counted, maintained, and tested for susceptibility. Materials used for larvae instar III reared up to F1. The study was carried out with 4 replications, using 25 larvae in each glass with a solution of temephos 0.02 mg/L in 250 mL of water and allowed to contact the test for 1 hour and the recovery period for 24 hours. The results obtained were 83.10% of the house contained eggs in the ovitrap and 16.90% had no eggs, the number of eggs in the ovitrap outside the home (57%) was more than inside the house (43%) and the ovitrap index obtained was 72, 5%, the percentage of deaths is 99% and is categorized as vulnerable.</em></p> <p><em> </em></p> <p> </p> </td> </tr> </tbody> </table> Iin Indriyani Emantis Rosa Gina Dania Pratami Nismah Nukmal Copyright (c) 2022 Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) https://creativecommons.org/licenses/by-sa/4.0 2022-01-06 2022-01-06 9 1 57 64 10.23960/jbekh.v9i1.205 Plant Species In The Honggoderpo Cemetery, Wonosobo Regency, Central Java http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/article/view/214 <p>Honggoderpo Cemetery is one of the urban green open spaces (RTH) which is located adjacent to the center of Wonosobo Regency, Central Java. This cemetery is a sacred area, so disturbances to the environment are rare, including plants. Therefore, plant diversity is still maintained...</p> Arifin Surya Dwipa Irsyam Hani Ristiawan Muhammad Rifqi Hariri Peniwidiyanti Peniwidiyanti Rina Ratnasih Irwanto Copyright (c) 2022 Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) https://creativecommons.org/licenses/by-sa/4.0 2022-01-06 2022-01-06 9 1 65 76 10.23960/jbekh.v9i1.214 The Ability of Soil Bacteria from Liwa Botanical Gardens to Produce Indole Acetic Acid Hormone (AIA) http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/article/view/202 <table> <tbody> <tr> <td width="300"> <p>Hormon AIA berperan besar dalam memperbesar dan memperpanjang sel, pembelahan sel, khususnya pada daerah ujung tanaman. Bakteri yang memiliki kemampuan dalam menghasilkan hormon AIA diantaranya <em>Enterobacter</em> sp., <em>Azospirillum</em> sp., <em>Klebsiella</em> sp., <em>Alcaligenes faecalis</em>, <em>Azoarcus</em> sp., <em>Serratia</em> sp., <em>Azotobacter</em> sp., <em>Cyanobacteria</em>, <em>Erwinia herbicola</em>, <em>Pseudomonas</em> sp., <em>Rhizobium</em>, <em>Bradyrhizobium</em>, <em>Agrobacterium</em> <em>tumafaciens</em>. Sintesis AIA ini memerlukan senyawa tambahan berupa triptopan. Namun beberapa bakteri mampu memproduksi AIA tanpa penambahan prekursor. Penelitian ini bertujuan untuk memperoleh isolat bakteri penghasil hormon AIA dari tanah Kebun Raya Liwa. Pada ini diawali dengan isolasi bakteri tanah asal Kebun Raya Liwa, selanjutnya dilakukan uji kemampuan produksi AIA secara kuantitatif tanpa penambahan triptopan. Pengujian produksi AIA secara kualitatif dilakukan dengan menggunakan media <em>Nutrien Broth</em> dengan metode spektrofotometri dengan penambahan reagen salkowsky dan dilakukan pengamatan setelah 72 jam inkubasi. Data yang didapatkan disajikan dalam bentuk tabel. Hasil penelitian didapatkan 9 isolat yang mampu menghasilkan hormon AIA dengan kadar yang berbeda. Isolat penghasil AIA tertinggi yaitu isolat DT1 dengan kadar 114 ppm yang mempunyai karakteristik berbentuk bacil berwarna putih susu dan tidak berspora.</p> <p> </p> <p> </p> </td> <td width="300"> <p><em>AIA hormone plays a role in enlarging and elongating cells, and cell division, especially at the tip of the plant. Bacteria that have the ability to produce IAA hormones include </em>Enterobacter<em> sp., </em>Azospirillum<em> sp., </em>Klebsiella<em> sp., </em>Alcaligenes faecalis<em>, </em>Azoarcus<em> sp., </em>Serratia<em> sp., </em>Azotobacter<em> sp., </em>Cyanobacteria<em>, </em>Erwinia herbicola<em>, </em>Pseudomonas<em> sp., </em>Rhizobium<em>, </em>Bradyrhizobium<em>, </em>Agrobacterium tumafaciens<em>. The synthesis of AIA requires an additional compound in the form of tryptophan. However, some bacteria can produce AIA without the addition of precursors. The goal of this study was to obtain isolates of AIA hormone-producing bacteria from the soil of the Liwa Botanical Gardens. This begins with the isolation of soil bacteria from the Liwa Botanical Gardens, then a quantitative test of AIA production capability is carried out without the addition of tryptophan. Qualitative testing of AIA production was carried out using Nutrien Broth media with spectrophotometric methods with the addition of Salkowsky reagent and observations were made after 72 hours of incubation. The data obtained are presented in tabular form. The results showed that 9 isolates were able to produce the AIA hormone at different levels. The highest AIA-producing isolate was the DT1 isolate with a concentration of 114 ppm which had the characteristics of a milky white bacillus and no spores. </em></p> <p><em> </em></p> <p> </p> <p><em> </em></p> </td> </tr> </tbody> </table> Cristina Nugroho Ekowati Agung Sanjaya Suratman Umar Sumardi Sumardi Copyright (c) 2022 Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) https://creativecommons.org/licenses/by-sa/4.0 2022-01-06 2022-01-06 9 1 77 82 10.23960/jbekh.v9i1.202 The Potential of Soil Bacterial Isolates from Liwa Botanical Gardens, West Lampung as Phosphate Solubilizing Bacteria http://jurnalbiologi.fmipa.unila.ac.id/index.php/jbekh/article/view/203 <table> <tbody> <tr> <td width="300"> <p>Tanah Kebun Raya Liwa (KRL) Lampung Barat merupakan tanah jenis laterit yang kandungan unsur hara dan kesuburan tanahnya rendah, sehingga tidak dapat mendukung pertumbuhan tanaman hias yang ada secara optimal. Salah satu unsur hara yang penting dalam menunjang kesuburan dan pertumbuhan tanaman adalah fosfat. Jumlah fosfat terlarut dalam tanah yang dapat dimanfaatkan oleh tanaman sangat sedikit sehingga menyebabkan defisiensi. Bakteri pelarut fosfat asli dibutuhkan untuk memenuhi kebutuhan fosfat bagi tanaman. Tujuan dari penelitian ini adalah untuk mendapatkan isolat bakteri pelarut fosfat yang berpotensi melarutkan fosfat. Penelitian ini dilaksanakan pada bulan Maret sampai Juni 2021 meliputi isolasi bakteri pelarut fosfat dan pengujian potensi pelarutan fosfat pada media pikovskaya padat. Potensi bakteri pelarut fosfat ditentukan berdasarkan nilai Indeks Kelarutan Fosfat (IPF) tertinggi. Hasil isolasi tanah dari Kebun Raya Liwa diperoleh 10 isolat yang mampu melarutkan fosfat dan menghasilkan zona bening. Isolat dengan potensi pelarutan fosfat terbaik adalah isolat C1 dan C3 dengan nilai indeks pelarutan fosfat 2, 6 dan 2.</p> <p> </p> <p> </p> <p> </p> </td> <td width="300"> <p><em>Liwa Botanical Gardens (KRL) soil, West Lampung is a lateritic soil type nutrient content and low soil fertility, so it can’t support the growth of existing ornamental plants optimally. One of the important nutrients in supporting fertility and plant growth is phosphate. The amount of dissolved phosphate in the soil that can be utilized by plants is very small, causing a deficiency. Indigenous phosphate solubilizing bacteria are needed to meet the needs of phosphate for plants. The purpose of this study was to obtain isolates of phosphate solubilizing bacteria that have the potential to dissolve phosphate. This research was carried out from March to June 2021 covering the isolation of phosphate solubilizing bacteria and testing the potential for dissolving phosphate on solid pikovskaya media. Potential phosphate solubilizing bacteria were determined based on the highest Phosphate Solubility Index (IPF) value. The results of soil isolation from Liwa Botanical Gardens obtained 10 isolates capable of dissolving phosphate and producing a clear zone. Isolates with the best phosphate solubilizing potential were isolates C1 and C3 with phosphate solubilizing index values of 2, 6 and 2.</em></p> <p><em> </em></p> <p> </p> <p><em> </em></p> </td> </tr> </tbody> </table> Cristina Nugroho Ekowati Rina Shintia Suratman Umar Bambang Irawan Copyright (c) 2022 Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) https://creativecommons.org/licenses/by-sa/4.0 2022-01-06 2022-01-06 9 1 83 89 10.23960/jbekh.v9i1.203