Size Shortening of Body Parts of Fetus Mice (Mus musculus L.) After Giving Bitter Melon Fruit Extract (Momordica charantia L.)

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Silvia Andriani
Nuning Nurcahyani
Hendri Busman
Sutyarso
Evi Kurniawaty
Mizan Sahroni
Egita Windrianatama Puspa

Keywords

Abstract

Bitter melon (Momordica charantia L.) is a plant that has traditional medicinal properties. In bitter melon fruit, there are several toxic compounds, namely momordicoside K and L, which are thought to have cytotoxic properties. This study aims to determine the teratogenic effect of bitter melon fruit extract given to pregnant females on the length of the fetus' cranium and sternum. Bitter melon fruit extraction was carried out using the maceration method using 95% ethanol. By using a completely randomized design 20 male mice and 20 female mice were divided into 4 groups. Each group consisting of 5 pregnant female mice was given bitter melon extract orally starting from day 6 to day 17 of pregnancy once a day in the morning with a treatment dose of aquabidest as a control [A], [B] 22.5 mg/30 gr BW, [C] 30 mg/30 gr BW, and [D] 37.5 mg/30 gr BW. Next, the female mice were dissected, the fetus was removed and the length of the cranium and the length of the sternum were measured. The results showed that in general, the administration of bitter melon fruit extract caused a decrease in the average length of the cranium and sternum. The results of the further analysis with the Least Significant Difference (LSD) at the 5% level showed that administering doses of [B], [C], and [D] had a significant effect on reducing the length of the cranium and sternum. The most effective dose to reduce the length of the cranium and sternum of fetal mice is 37.5 mg/30 g BW

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References

[1] P. Bolouri et al., “Applications of Essential Oils and Plant Extracts in Different Industries,” Molecules, vol. 27, no. 24, p. 8999, Dec. 2022, doi: 10.3390/molecules27248999.
[2] Z. Asyikinbinti et al., “Application of Herbal extract and its medicinal value”, [Online]. Available: www.scholarsresearchlibrary.com
[3] S. Hosseinzadeh, A. Jafarikukhdan, A. Hosseini, and R. Armand, “The Application of Medicinal Plants in Traditional and Modern Medicine: A Review of <i>Thymus vulgaris</i>,” Int J Clin Med, vol. 06, no. 09, pp. 635–642, 2015, doi: 10.4236/ijcm.2015.69084.
[4] M. Alqamari, D. M. Tarigan, and Alridwirsah, Budidaya Tanaman Obat. Medan: Umsu Press, 2017.
[5] N. Wathoni, A. Haerani, N. Yuniarsih, and R. Haryanti, “A review on herbal cosmetics in Indonesia,” International Journal of Applied Pharmaceutics, vol. 10, no. 5. Innovare Academics Sciences Pvt. Ltd, pp. 13–16, 2018. doi: 10.22159/ijap.2018v10i5.28102.
[6] S. Jia, M. Shen, F. Zhang, and J. Xie, “Recent advances in Momordica charantia: Functional components and biological activities,” International Journal of Molecular Sciences, vol. 18, no. 12. MDPI AG, Dec. 01, 2017. doi: 10.3390/ijms18122555.
[7] N. Ahmad, N. Hasan, Z. Ahmad, M. Zishan, and S. Zohrameena, “Journal of Drug Delivery and Therapeutics An International Peer Reviewed Journal Open access to Pharmaceutical and Medical research Momordica charantia: FOR TRADITIONAL USES AND PHARMACOLOGICAL ACTIONS,” Journal of Drug Delivery & Therapeutics, vol. 6, no. 2, p. 40, 2016, [Online]. Available: http://jddtonline.info
[8] Satya Vani Chekka and Naresh Kumar Mantipelly, “Momordica charantia: A natural medicinal plant,” GSC Biological and Pharmaceutical Sciences, vol. 12, no. 2, pp. 129–135, Aug. 2020, doi: 10.30574/gscbps.2020.12.2.0251.
[9] K. S. Gayathry and J. A. John, “A comprehensive review on bitter gourd (Momordica charantia L.) as a gold mine of functional bioactive components for therapeutic foods,” Food Production, Processing and Nutrition, vol. 4, no. 1. BioMed Central Ltd, Dec. 01, 2022. doi: 10.1186/s43014-022-00089-x.
[10] V. E. Villarreal-La Torre, W. S. Guarniz, C. Silva-Correa, L. Cruzado-Razco, and R. Siche, “Antimicrobial activity and chemical composition of
Momordica charantia: A review,” Pharmacognosy Journal, vol. 12, no. 1. E Manuscript Technologies, pp. 213–222, 2020. doi: 10.5530/pj.2020.12.32.
[11] Mangkoewidjojo and Smith, Pemeliharaan, pembiakkan, dan penggunaan hewan percobaan di Daerah Tropis. Jakarta: UI Press, 1988.
[12] G. A. Silvia, “Pengaruh suspensi sari akar manis terhadap perkembangan janin pada mencit bunting ,” Indonesia University, Jakarta, 2011.
[13] S. K. Thiagarajan, K. Rama Krishnan, T. Ei, N. Husna Shafie, D. J. Arapoc, and H. Bahari, “Evaluation of the Effect of Aqueous Momordica charantia Linn. Extract on Zebrafish Embryo Model through Acute Toxicity Assay Assessment,” Evidence-based Complementary and Alternative Medicine, vol. 2019, 2019, doi: 10.1155/2019/9152757.
[14] D. Pharma, M. R. Mari Santos, A. R. Agpaoa, A. D. Sayson, M. G. Ellenita De Castro, and R. R. Milton Dulay, “ISSN 0975-413X CODEN (USA): PCHHAX Toxic and Teratogenic Effects of Water Leaf Extract of Momordica charantia in Zebrafish (Danio rerio) Embryos,” 2017. [Online]. Available: www.derpharmachemica.com
[15] M. F. Khan, N. Abutaha, F. A. Nasr, A. S. Alqahtani, O. M. Noman, and M. A. M. Wadaan, “Bitter gourd (Momordica charantia) possess developmental toxicity as revealed by screening the seeds and fruit extracts in zebrafish embryos,” BMC Complement Altern Med, vol. 19, no. 1, p. 184, Dec. 2019, doi: 10.1186/s12906-019-2599-0.
[16] V. Perumal et al., “Toxicity and teratogenicity evaluation of ethanolic extract from Momordica charantia fruit using zebrafish (Danio rerio) embryo model,” Int Food Res J, vol. 29, no. 3, pp. 531–539, 2022, doi: 10.47836/ifrj.29.3.06.