Histochemical Analysis of Secondary Metabolite Compounds in Zinnia elegans from Tambakaji, Ngaliyan, Semarang, Central Java Uji Kandungan Senyawa Histokimia pada Tumbuhan Zinnia elegans di Tambakaji Ngaliyan Semarang Jawa Tengah
Main Article Content
Abstract
The presence of Zinnia elegans in the Tambakaji area has so far been viewed primarily from an aesthetic perspective, despite its biological potential through secondary metabolite content that requires further investigation. This study critically determines the localization of secondary metabolite compounds in various plant organs to map the pharmacological capacity of the species. The study was conducted using a qualitative descriptive approach with fresh preparation techniques and specific reagents. The observation results showed an organ-specific distribution of secondary metabolite; alkaloids, phenols, and tannins were strongly identified in root and stem tissues, while flavonoids and lipids were not detected in any organ. These findings indicate that the distribution of secondary metabolite in Zinnia elegans is not evenly spread across all organs. This study provides fundamental information for the utilization of Zinnia elegans as a source of natural bioactive raw materials and serves as an important reference for the development of its pharmacological potential.
Downloads
Article Details

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
1] A. S. Tugbaeva, A. A. Ermoshin, H. Wuriyanghan, and I. S. Kiseleva, “Lignification in Zinnia (Zinnia elegans Jacq.) Stem Sections of Different Age : Biochemical and Molecular Genetic Traits,” Horticulturae, vol. 9, no. 3, pp. 1–14, 2023, doi: https://doi.org/10.3390/horticulturae9030410.
[2] Y. I. Yahya, “Pemanfaatan Turnera subulata dan Zinnia elegans sebagai Tanaman Refugia terhadap Kelimpahan Arthropoda pada Tanaman Bawang Merah (Allium ascalonicum),” Universitas Muhammadiyah Sumatera Utara, 2020.
[3] Z. Xu, H. Zhang, X. Chen, and C. Zhou, “Soil environment , physiological metabolism and ornamental traits of Zinnia response to water and nitrogen combination in a cold and arid environment,” Front. Plant Sci., vol. 17, no. March, pp. 1–21, 2026, doi: 10.3389/fpls.2026.1785249.
[4] X. Sun et al., “Environmental Response to Root Secondary Metabolite Accumulation in Paeonia lacti fl ora : Insights from Rhizosphere,” Microbiol. Spectr., vol. 10, no. 6, pp. 1–20, 2022.
[5] A. F. Burlec et al., “Chemical Profile and Antioxidant Activity of Zinnia elegans Jacq. Fractions,” Molecules, vol. 24, no. 16, pp. 1–16, 2019, doi: https://doi.org/10.3390/molecules24162934.
[6] J. Qian, L. Jiang, H. Qing, J. Fu, and C. Zhang, “ZeMYB9 regulates cyanidin synthesis by activating the expression of fl avonoid 3 ′ -hydroxylase gene in Zinnia elegans,” Front. Plant Sci., vol. 13, no. October, pp. 1–16, 2022, doi: 10.3389/fpls.2022.981086.
[7] N. Sofiyanti, D. Iriani, and A. R. Lestari, “Kajian Anatomi-Histokimia Tangkai Daun dan Karakteristik Epidermis Pucuk Merah (Syzygium myrtifolium Walp. – Myrtaceae),” Bul. Anat. dan Fisiol., vol. 7, no. 2, pp. 83–90, 2022.
[8] N. Agustina and N. Istiqomah, “Analisis Kadar Metabolit Sekunder, Histokimia, dan Aktivitas Antioksidan Akar Acalypha indica L.,” J. Syifa Sci. Clin. Res., vol. 3, no. 2, pp. 45–51, 2021.
[9] P. P. Mitra and D. Loqué, “Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements,” J. Vis. Exp. JoVE, no. May, pp. 1–11, 2014, doi: 10.3791/51381.
[10] K. Doolabh, Y. Naidoo, and Y. H. Dewir, “Micromorphology, Ultrastructure and Histochemistry of Commelina benghalensis L. Leaves and Stems,” Plants, vol. 10, no. 3, pp. 1–17, 2021.
[11] V. Yadav et al., “Histochemical Techniques in Plant Science : More Than Meets the Eye,” Plant Cell Physio, vol. 62, no. 10, pp. 1509–1527, 2021.
[12] M. Maisarah, M. Chatri, and L. Advinda, “Characteristics and Functions of Alkaloid Compounds as Antifungals in Plants Karakteristik dan Fungsi Senyawa Alkaloid sebagai Antifungi pada Tumbuhan Abstrak Meode Penelitian,” Serambi Biol., vol. 8, no. 2, pp. 231–236, 2023.
[13] Z. Wu, R. Wang, Z. Sun, Y. Su, and L. Xiao, “A mass spectrometry imaging approach on spatiotemporal distribution of multiple alkaloids in Gelsemium elegans,” Plants Sci., vol. 13, no. November, pp. 1–15, 2022, doi: 10.3389/fpls.2022.1051756.
[14] M. A. S. R. H. Sianipar, “Pemeriksaan senyawa alkaloid pada beberapa tanaman familia solanaceae serta identifikasinya dengan kromatografi lapis tipis (KLT),” J. Farmanesia, no. Vol 4 No 1 (2017): Jurnal Farmanesia, pp. 1–11, 2017, [Online]. Available: http://e-journal.sari-mutiara.ac.id/index.php/2/article/view/257/262
[15] V. M. Hartoyo, B. R. Sidharta, and S. S. Widiastuti, “Artikel aktivitas antibakteri ekstrak daun bandotan (Ageratum conyzoides) terhadap Bacillus cereus dan Salmonella typhi,” Ber. Biol., vol. 24, no. April, pp. 37–49, 2025, doi: 10.55981/berita.
[16] R. R. Maulidya, R. Saputri, H. Liana, and F. Hasymi, “Uji Aktivitas Antioksidan Ekstrak Etil Asetat Daun Tigaron (Crateva Religiosa) Menggunakan Metode DPPH,” Borneo J. Pharmascientech, vol. 07, no. 02, pp. 110–121, 2023.
[17] Suratno, “Skrining fitokimia ekstrak etanol mikroalga,” J. Surya Med., vol. 1, no. 2, pp. 26–33, 2010.
[18] S. N. Ika and N. Herdyastuti, “Pengaruh suhu terhadap kandungan fenolik total dan aktivitas antioksidan bawang putih bubuk dan bawang hitam bubuk,” UNESA J. Chem., vol. 10, no. 3, pp. 348–355, 2021.
[19] S. Syamsudin, A. H. Alimuddin, and B. Sitorus, “Isolasi dan karakterisasi senyawa fenolik dari daun putat (Planchonia valida Blume),” Indones. J. Pure Appl. Chem., vol. 5, no. 2, pp. 85–98, 2022.
[20] S. Sunani and R. Hendriani, “Review Article : Classification and Pharmacological Activities of Bioactive Review Jurnal : Klasifikasi dan Aktivitas Farmakologi dari Senyawa Aktif,” Indones. J. Biol. Pharm., vol. 3, no. 2, pp. 130–136, 2023.
[21] B. Halimu, R. S. Sulistijowati, and L. Mile, “Identifikasi Kandungan Tanin pada Sonneratia Alba,” NikèJurnal Ilm. Perikan. dan Kelaut., vol. 5, no. 4, pp. 93–97, 2017.
[22] I. E. Dafrita and M. Sari, “Senduduk dan ubi jalar ungu sebagai pewarna preparat squash akar bawang merah CHECK,” JPBIO ( J. Pendidik. Biol. ), vol. 5, no. 1, pp. 46–55, 2020, doi: 10.31932/jpbio.v5i1.571.
[23] N. Febriadi, Nofisulastri, and A. Sukri, “Studi penggunaan safranin dan kolkisin dalam pengamatan kromosom pada sel akar bawang bombay (Allium cepa Var) Noval,” Biosci. J. Ilm. Biol., vol. 11, no. 1, pp. 839–846, 2023.
[24] R. Noor, N. Y. Tika, and P. Agustina, “Preparat jaringan tumbuhan dengan menggunakan pewarna alami sebagai media belajar jaringan tumbuhan,” J. Lentera Pendidik. Pus. Penelit. LPPM UM METRO, vol. 5, no. 2, pp. 136–148, 2020.
[25] R. Azlina and D. Iriani, “Analisis Struktur Anatomi dan Histokimia Daun Tanaman Buah Makasar (Brucea javanica (L.) Merr.),” Bul. Anat. dan Fisiol., vol. 9, no. 2, pp. 137–147, 2024.
[26] K. Prashariska and A. Pitoyo, “Pengaruh indole-3-acetic acid (IAA) dan benzyl amino purine (BAP) terhadap induksi dan deteksi alkaloid kalus kamilen (Matricaria chamomilla L.),” Innofarm J. Inov. Pertan., vol. 23, no. 2, pp. 104–114, 2021.
[27] B. A. Ivanda, “Perbandingan Struktur Anatomi dan Uji Histokimia Kemukus (Piper cubeba L . f .) dan Kemukus Semu (Piper lanatum Roxb.),” Universitas Islam Negeri Walisongo, 2023.
[28] Nurhasanah and D. Iriani, “Histochemical Test of root, petiole and leaf of Kelembak (Rheum officinale Baill.),” J. Biol. Trop., vol. 21, no. 3, pp. 726–733, 2021.
[29] L. A. Hermawan, S. Husna, and H. A. Akmalia, “Identifikasi Senyawa Metabolit pada Organ Akar dan Batang Kangkung Air ( Ipomoea aquatica Forssk .) Berdasarkan Uji Histokimia Identification of Metabolite Compounds in Root and Stem Organs of Water Spinach ( Ipomoea aquatica Forssk .) Based on Histochemi,” LenteraBio, vol. 12, no. 3, pp. 317–322, 2023.
[30] R. Mahmud, “Uji aktivitas antioksidan ekstrak ampas sagu (Metroxylon sagu rottb) di Desa Pangi Kecamatan Dulupi Kabupaten Boalemo,” J. Ilm. dr. Aloei Saboe, vol. 2, no. 1, pp. 23–33, 2022.